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Campana,Eloiza Helena; Xavier,Danilo Elias; Petrolini,Fernanda Villas-Boas; Cordeiro-Moura,Jhonatha Rodrigo; Araujo,Maria Rita Elmor de; Gales,Ana Cristina. |
Abstract The mechanisms involved in the uncommon resistance phenotype, carbapenem resistance and broad-spectrum cephalosporin susceptibility, were investigated in 25 Pseudomonas aeruginosa clinical isolates that exhibited this phenotype, which were recovered from three different hospitals located in São Paulo, Brazil. The antimicrobial susceptibility profile was determined by CLSI broth microdilution. β-lactamase-encoding genes were investigated by PCR followed by DNA sequencing. Carbapenem hydrolysis activity was investigated by spectrophotometer and MALDI-TOF assays. The mRNA transcription level of oprD was assessed by qRT-PCR and the outer membrane proteins profile was evaluated by SDS-PAGE. Genetic relationship among P. aeruginosa isolates was assessed... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gram-negative bacilli; Carbapenem resistance; OprD porin. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100057 |
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Araujo,Maria Rita Elmor de; Aquino,Caroline; Scaramal,Elton; Ciola,Claudete Sílvia; Schettino,Guilherme; Machado,Marcel Cerqueira Cesar. |
Non cholera Vibrio may cause conjunctivitis, wound infection, gastroenteritis and serious sepsis. Transmission to men is through contact with skin, mucosa or wounds exposed to marine water, and consumption of certain barely cooked or raw seafood, more frequently in the summer. This is one of the first cases of severe infection related to Vibrio vulnificus described in Brazil. The patient was an old man, who ingested seafood in Guarujá, a seashore city near São Paulo, 3 days before hospitalization. He was admitted to the emergency room in an ill state with septic shock. On 2 sets of blood culture a highly virulent microorganism was isolated, Vibrio vulnificus, which leads to sepsis and frequently to death in susceptible patients. The objective of this... |
Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Vibrio vulnificus; Septic shock; Risk factors. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200029 |
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